Fig. 2.
Backbone dynamics of nsp10. a Schematic representation of an NMR chemical-shift-based TALOS-N secondary-structure prediction of nsp10. b {1H}-15N heteronuclear NOEs measured at 298 K and 0.15 mM nsp10 are shown as a function of residue number. c The temperature coefficients (Tcoeff) determined from a series of 2D 1H,15N BEST-TROSY spectra measured from 293 to 308 K with 5 K increments at 600 MHz. d Overlay of 2D 1H,15N BEST-TROSY spectra recorded at 600 MHz at different temperatures (color code is shown in the figure). e Selected zoom-in showing residues A32, C86, A116 and C140, representing different hydrogen bond strengths. f Plot of the amide proton chemical shift (in ppm) as a function of temperature (in K)