Table 1.
List of experiments collected to perform the sequence specific assignment of nsp10. Main parameters used are reported
| Experiments | Time domain data size (points) | Spectral width (ppm) | Number of scans | Delay time (s) | NUS % | ||||
|---|---|---|---|---|---|---|---|---|---|
| T1 | T2 | T3 | F1 | F2 | F3 | ||||
| 1H,15N BEST-TROSY | 1024 | 1272 | 50 (15N) | 10.0 (1H) | 4 | 0.3 | |||
| BEST-TROSY-HN(CO)CACB | 208 | 256 | 1272 | 59.7 (13C) | 26.0 (15N) | 10.0 (1H) | 8 | 0.3 | |
| BEST-TROSY-HNCACB | 216 | 256 | 1272 | 59.7 (13C) | 26.0 (15N) | 10.0 (1H) | 8 | 0.3 | |
| BEST-TROSY-HN(CA)CO | 216 | 256 | 1272 | 12.0 (13C) | 26.0 (15N) | 10.0 (1H) | 8 | 0.3 | 25 |
| BEST-TROSY-HNCO | 216 | 256 | 1272 | 12.0 (13C) | 26.0 (15N) | 10.0 (1H) | 4 | 0.3 | |
| 15N R1 | 12 | 256 | 2048 | – | 28.0 (15N) | 16.0 (1H) | 8 | 2.0 | |
| 15N R2 | 12 | 256 | 2048 | – | 28.0 (15N) | 16.0 (1H) | 8 | 2.0 | |
| 15N-NOE | 2 | 256 | 2048 | – | 28.0 (15N) | 16.0 (1H) | 80 | 10 | |
| TRACT | 28 | 96 | 896 | – | 30.0 (15N) | 12.0 (1H) | 8–40 | 0.3 | |