Figure 2.
miR-1297 regulates the aerobic glycolysis of OS cells. (A and B) Extracellular acidification rate (ECAR) of OS cells lines (MNNG-HOS and U-2OS cells) in control and miR-1297 overexpression group was varied by using a Seahorse Bioscience XFp analyzer. (C and D) O2 consumption rate (OCR) of OS cells lines (MNNG-HOS and U-2OS cells) in control and miR-1297 overexpression group was detected by using a Seahorse Bioscience XFp analyzer. O: Oligomycin, F: FCCP, A&R: antimycin A/rotenone. (E and F) Lactate production and ATP level was determined in the OS cells (MNNG-HOS and U-2OS cells) stable knockdown miR-1297. Values are means ± SD, *p<0.05.
Abbreviations: Glc, glucose; Oligo, oligomycin; 2-DG, 2-deoxy-d-glucose.
