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. 2020 Nov 3;13:11265–11275. doi: 10.2147/OTT.S274744

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© 2020 Pan et al.

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Upregulating of PFKFB2 partly compensates the effect of silencing of miR-1297 in OS. (A) The efficacy of overexpression of PFKFB2 in miR-1297 overexpression OS cells (MNNG-HOS and U-2OS) were confirmed by Western blotting. (B and C) Overexpression of PFKFB2- partly reversed the inhibitory effects of miR-1297 overexpression on the CCK8 assay of MNNG-HOS and U-2OS cells, values are means ± SD, **p < 0.01. (D and E) PFKFB2-overexpression partly reversed the inhibitory effects of miR-1297 overexpression on the colony formation properties of MNNG-HOS and U-2OS cells, values are means ± SD, **p < 0.01. (F and G) PFKFB2-overexpression partly reversed the induce effects of miR-1297 overexpression on apoptosis of MNNG-HOS and U-2OS cells. Values are means ± SD, **p<0.01. (H and I) Altered level of ECAR and OCR in MNNG-HOS and U-2OS cells in different groups (Control, ov-miR-1297, ov-miR-1297 and ov-PFKFB2). Values are means ± SD. (J and K) Lactate production and ATP level was determined in different groups. Values are means ± SD, *p<0.05.

Abbreviations: Glc, glucose; Oligo, oligomycin; 2-DG, 2-deoxy-d-glucose.