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. 2020 Nov 3;12:11169–11181. doi: 10.2147/CMAR.S272274

Figure 5.

Figure 5

SHMT2 overexpression reverses the anticancer functions of si-circ_0072995 in breast cancer cells. (A) Western blot analysis of SHMT2 protein expression in SKBR3 and BT-549 cells transfected with SHMT2 or vector. SKBR3 and BT-549 cells were transfected with si-NC, si-circ#3, si-circ#3 + vector, or si-circ#3 + SHMT2. (B) CCK-8 assay analysis of SKBR3 and BT-549 cell proliferation. (C) Caspase-3 activity analysis of SKBR3 and BT-549 cells using a colorimetric assay kit. (D) The analysis of SKBR3 and BT-549 cell apoptosis using flow cytometry. (E and F) The analysis of the migratory and invasive capabilities of SKBR3 and BT-549 cells using transwell assay. (G) Cell adhesion ability analysis in SKBR3 and BT-549 cells using cell adhesion assay. (H–J) Detection of ATP levels, glucose uptake, and lactate production in SKBR3 and BT-549 using an ATP, glucose, or lactate assay kit, respectively. (K and L) Western blot analysis of HK-2, LDHA, and GLUT1 protein in SKBR3 and BT-549 cells. *P<0.05.