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. 2020 Nov 3;12:11169–11181. doi: 10.2147/CMAR.S272274

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© 2020 Qi et al.

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MiR-149-5p directly binds to circ_0072995 or SHMT2 in breast cancer cells. (A) Schematic illustration exhibiting overlapping of the target miRNAs of circ_0072995 and SHMT2 predicted by online databases (circBase, circInteractome, TargetScan, starbase, miRwalk and miRDB), (B) The predicted binding sites of miR-149-5p on circ_0072995 or SHMT2 sequences. (C and D) Interaction analysis between miR-149-5p and circ_0072995 or SHMT2 in SKBR3 and BT-549 cells using the dual-luciferase reporter assay. (E) qRT-PCR analysis of miR-149-5p expression in SKBR3 and BT-549 cells transfected with si-NC or si-circ#3. (F) qRT-PCR analysis of miR-149-5p expression in SKBR3 and BT-549 cells transfected with miR-149-5p or miR-NC. (G) Western blot analysis of SHMT2 protein expression in SKBR3 and BT-549 cells transfected with miR-149-5p or miR-NC. *P<0.05.