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. 2020 Sep 17;216(3):689–700. doi: 10.1534/genetics.120.303642

Figure 3.

Figure 3

Transcription termination causes extension of the H3K27me3 domain at tou. (A) ChIP-seq distributions of H3K27me3 (blue), H3K36me3 (green), and CP190 (aqua) in WT and tou-MiMIC. The tou gene isoforms are shown at the bottom (navy blue). The transgene insertion site in tou-MiMIC is indicated with a vertical black line. (B) ChIP-seq profiles of H3K27me3 (rows 1 and 3) and H3K36me3 (rows 2 and 4) in WT and tou-MiMIC in a custom gene track that includes the MiMIC transgene sequences. The transgene is not present in WT (red box with an X). The locations of the tou gene and transcription units are shown at the bottom along with the MiMIC transgene. The scale is different in (B) (0–800) because it is reads per million in only a 400 kb region including the tou-MiMIC transgene and surrounding sequences, rather than the entire genome, as it is in both (A) and other figures in this paper.