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. 2020 Sep 17;216(3):689–700. doi: 10.1534/genetics.120.303642

Figure 5.

Figure 5

The inv-en “ON” state extends to the tou-MiMIC insertion site. (A) Top panel: inv-en region and two imaginal disc enhancers [IDEs, coordinates- 2R:7435274-7439183 and 2R:7448808-7455476, dm5]. MiMIC insertion sites are indicated with black lines. Bottom panel: yellow gene expression from the MiMIC transgenes in wing imaginal discs by RNA FISH with y probe. Regions where the yellow gene is expressed in tou-MiMIC are indicated with red arrowheads. (B) Schematic of the eZ-λ-eG construct that replaced the MiMIC element in tou. Blue boxes: eve promoter (eve pr) and eve 3′ untranslated region and poly(A) addition signal (eve utr). Black box: ∼0.5 kb bacteriophage λ DNA insert. (C) Immunostaining of GFP and β-galactosidase (β-gal) proteins in wing imaginal discs (anterior to the left) and embryos (bottom) (stage 13, anterior left, dorsal up). eZ-λ-eG was inserted in both orientations into the tou-MiMIC site (orientation shown above). Note that the GFP and β-gal expression in wing discs is stronger in the same cells that express y in tou-MiMIC (red arrowheads).