Figure 5.

Overexpression of ovhts results in larger ring canals. (A–F) Fluorescence micrographs of F-actin staining and HtsRC antibody labeling in egg chambers throughout oogenesis show that maternal alpha tubulin-GAL4 (matGal4)-driven UASH-ovhts::GFP leads to the formation of large ring canals, compared to w¹¹¹⁸ control. Insets contain higher-resolution micrographs of nurse cell and oocyte ring canals from the same egg chamber. Inset scale bars are 10 μm. (G) Quantification of ring canal diameter throughout development, visualized by violin plots. Refer to Table S1 for information about the means and sample numbers for the data in this figure panel. Yellow dotted lines indicate median ring canal diameter, and black dotted lines represent the upper and lower quartiles. Welch’s t-test was performed between w¹¹¹⁸ control and matGal4>ovhts::GFP ring canal diameters at each stage; Significance thresholds: **P < 0.01; ****P < 0.0001.