Figure 6.

Ectopic HtsRC expression drives the formation of F-actin aggregates. (A–F) Stage 10B egg chambers focusing on the somatic follicle cell layer surrounding the oocyte either from the basal surface (A, B, D, and E) or from the side with the basal side at the bottom and the apical side and germline at the top (C and F). (A–C) Wild-type (w¹¹¹⁸) control egg chamber labeled with Phalloidin and HtsRC antibody. All images shown were taken from the same egg chamber. (D–F) Ectopic expression of UASh-ovhts::APEX::V5 driven with the tubulin-Gal4 (tubGal4) driver. All images are taken from the same egg chamber. (G–H) tubulin-Gal4 driving UASh-ovhts::GFP along with either UASp-mCherry::kelch or a UASp-mCherry control. (I) Total area occupied by foci in a Z-projection the follicle cells, measured by HtsRC::GFP fluorescence. (J) Total HtsRC::GFP fluorescence contained within aggregates in control or UASp-mCherry::kelch overexpression. (I and J) Student’s t-tests were used to determine significance. Significance thresholds: *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001; ns, not significant.