Fig. 2.

Protocol followed for the blinded chronic i.p. administration of vehicle (30% SBE-β-cyclodextrin) and JNJ-47965567. Animals were trained for 3 consecutive days in the RotaRod apparatus just before the commencement of the treatment. At P60, mice were divided into two treatment groups (Vehicle or JNJ-47965567) and administrations continued 4 times per week until the humane endpoint. Disease progression was followed according to the protocol by assessing motor coordination with RotaRod, by measuring body weight and according to the phenotypic NeuroScore test (see Materials and Methods). At the humane endpoint, mice were euthanized, and spinal cords were dissected and stored until used. All tests were blinded for the investigators involved.