Fig. 2.

An extended 3′ sequence is required for the efficient generation of scaRNA fragments. DNA constructs expressing wild-type (WT) or 3′ deletion (Δ) scaRNA2 or scaRNA17 were transfected into HeLa cells followed by RNA isolation, Northern blotting and detection with probes described in Fig. 1. Full-length (FL) scaRNA 2 and 17 and fragments thereof are indicated. Endogenous signals are present in lanes containing RNA from untransfected cells (Endo). Primary-scaRNA signal is denoted (Pri). The data shown in the gels was quantified and displayed in histograms. Specifically, the fragment signal was divided by the FL+Pri signal and the WT value was set to 1. Both the FL+Pri signals were included in the calculation in order to account for all ectopic expression products.