Fig. 3.

Intronic sequences are required for the efficient processing of scaRNA9. (A) Schematic of scaRNA9 constructs illustrating the absence of 5′ intronic sequence in the 3′ extension construct (3′ ext) and the lack of both 5′ and 3′ intronic sequences in the deletion construct (Δ). (B) Detection of full-length (FL) scaRNA9 and the mgU2-30 fragment by Northern blot using RNA from untransfected HeLa cells (Endo) or HeLa cells ectopically-expressing WT, 3′ ext, or Δ scaRNA9. Primary-scaRNA signal is denoted (Pri). The data shown in the gel was quantified (histogram). Specifically, the fragment signal was divided by the FL+Pri signal and the WT value was set to 1. Both the FL+Pri signals were included in the calculation in order to account for all ectopic expression products.