Skip to main content
. 2020 Oct 26;9(10):bio054619. doi: 10.1242/bio.054619

Fig. 6.

Fig. 6.

The CNNC domains impact in vivo mgU2-30 biogenesis. (A) Schematic of scaRNA9 constructs annotating the three CNNC domains found downstream of the mgU2-30 fragment and the mutations that replace or delete these domains in mutants #3, #4, and #5. (B) Northern blot detection of the mgU2-30 fragment and FL scaRNA9 from RNA ectopically-expressing the WT, or mutant #3, #4, or #5 scaRNA9 construct. (C) Histogram generated from the quantification of data shown in (B). For each condition, the mgU2-30 signal was divided by the FL scaRNA9 signal in that lane, and normalized to that obtained from WT. N=4 biological repeats, **=P<0.0025, ****=P<0.0001, error bars represent standard deviation. (D) Northern blot detection of RNA isolated from in vitro processing assays with Drosha/DGCR8 using WT and mutant #3, #4 and #5 scaRNA9 constructs as substrates. The mgU2-30 fragment generated by the in vitro processing assay is denoted.