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. 2020 Nov 6;10:19237. doi: 10.1038/s41598-020-75930-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Response of epithelial cell cultures to the presence of purified BL23 EVs. (A) Cell viability measured using the resazurin reduction assay after incubation of HT-29 #16 cells with different concentrations of BL23 EVs (20 ng/ml to 10 µg/ml), IL1-β and TNF-α. (B) HT-29 #16 cells bearing phosphatase activity under the control of NF-kB were treated for 24 h with different concentrations of BL23 EVs (20 ng/ml to 10 µg/ml) and IL-1β and TNF-α (both 10 ng/ml). Cultured cells were collected and the reporter gene for secreted alkaline phosphatase activity was measured. Statistical analysis was performed using one-way ANOVA test, ***p < 0.001 compared to Control using Dunnett’s post test and student’s t-test, ^#p < 0.05, ^## < 0.001 compared to Control.