Table 3.
Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of liposomes with besifloxacin against S. epidermidis and P. aeruginosa planktonic cells.
| Treatments | S. epidermidis | P. aeruginosa | ||
|---|---|---|---|---|
| MIC (μg/mL) | MBC (μg/mL) | MIC (μg/mL) | MBC (μg/mL) | |
| Besifloxacin | 0.156 ± 0.0 | 0.156 ± 0.0 | 0.625 ± 0.0 | 0.625 ± 0.0 |
| LP PC | 0.156 ± 0.0 | 0.156 ± 0.0 | 0.625 ± 0.0 | 0.625 ± 0.0 |
| LP PC:SPM | 0.156 ± 0.0 | 0.156 ± 0.0 | 0.625 ± 0.0 | 0.625 ± 0.0 |
| Besivance | 0.156 ± 0.0 | 0.156 ± 0.0 | 1.25 ± 0.0 | 1.25 ± 0.0 |
| LP PC vehicle control | All viable | All viable | All viable | All viable |
| LP PC:SPM vehicle control | All viable | All viable | All viable | All viable |
| pH 11 HEPES solution | All viable | All viable | All viable | All viable |
| Viability control | All viable | All viable | All viable | All viable |
| Sterility control | Inviable | Inviable | Inviable | Inviable |
Data are expressed as mean ± standard deviation (n = 3). Treatments were besifloxacin in solution at 500 µg/mL; LP PC containing 467.84 ± 2.37 µg/mL of besifloxacin; LP PC:SPM containing 335.81 ± 8.28 µg/mL of besifloxacin; Besivance: commercial formulation diluted in HEPES buffer pH 7.4 to 500 µg/mL of the drug; LP PC vehicle control without drug; LP PC:SPM vehicle control without drug; pH 11 HEPES solution: vehicle control of the drug; Viability control: strains of S. epidermidis and P. aeruginosa; Sterility control: culture medium.