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. 2020 Nov 6;10:19242. doi: 10.1038/s41598-020-76186-z

Figure 5.

Figure 5

Plaque-forming efficiencies of B. pseudomallei wild-type or plc mutants in HeLa cells. (a) Representative images of plaques. (b) Plaque-forming efficiency of either B. pseudomallei wild-type K96243, plc1, plc2, plc3, plc12 or plc123 mutants in HeLa cells infected at an MOI of 10. Plaques were visualized by crystal violet staining of the monolayers at 24 h p.i. Error bars represent standard error of the mean for data collected in 3 independent experiments. Asterisks indicate significant differences (**P < 0.01, and ***P < 0.001, one-way ANOVA followed by Dunnett's post hoc test) between B. pseudomallei wild-type K96243 and mutant strains. The figure was prepared using GraphPad Prism version 7.05 for Windows (www.graphpad.com).