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. 2020 Aug 24;45(8):675–685. doi: 10.1093/chemse/bjaa056

Table 2.

Analysis of the brief-access lick data in Figure 1

Latency to initiate licking Lick rate
Test solutions Source of variation F-ratio df P-value F-ratio df P-value
E and water Test solution 22.8 1, 15 <0.0003 78.9 1, 15 <0.0001
Consecutive trials 2.4 4.5, 67.2 0.051 7.6 4.4, 66.3 <0.0001
Interaction 0.9 4.8, 72.6 0.515 12.9 4.7, 70.2 <0.0001
E and E + SS Test solution 11.9 1, 15 <0.005 20.9 1, 15 <0.001
Consecutive trials 1.3 5.3, 80.6 0.279 3.0 5.0, 75.3 0.016
Interaction 1.2 4.7, 69.9 0.287 1.2 3.9, 59 0.300
E and E + SSM Test solution 2.1 1, 15 0.173 10.4 1, 15 0.006
Consecutive trials 2.8 2.2, 33.5 0.067 6.4 4.7, 71.1 <0.0001
Interaction 1.1 3.8, 57.1 0.350 4.0 5.5, 81.9 0.002
E + SS and E + SSM Test solution 3.2 1, 11 0.102 27.0 1, 11 0.0003
Consecutive trials 1.9 5.9, 54.9 0.105 7.0 3.3, 36.1 0.0006
Interaction 1.6 5.4, 59.6 0.158 0.7 2.4, 26.9 0.519
E and E + SSM2 Test solution 13.5 1, 15 0.002 123.1 1, 15 <0.0001
Consecutive trials 1.8 5.4, 81.3 0.114 5.8 4.9, 73.5 0.0002
Interaction 1.4 5.8, 88.1 0.223 8.4 4.7, 70.3 <0.0001
E and E + SSM4 Test solution 27.2 1, 15 0.0001 42.4 1, 15 <0.0001
Consecutive trials 0.9 4.4, 66.5 0.467 0.9 3.9, 58.7 0.449
Interaction 0.3 4.6, 68.7 0.877 1.4 2.9, 44.1 0.265

In each lick test, the rat was offered two test solutions. We examined how (a) latency to initiate licking and (b) lick rate for each test solution changed across the initial 20 trials of the lick test (mean ± standard error). We alternated the presentation of each of the test solution, resulting in a total of 10 trials per test solution. For each dependent measure and pair of test solutions, we ran a 2-way repeated-measure ANOVA. We controlled for sphericity by adjusting dfs with the Greenhouse–Geisser correction.