Figure 5. Signaling through HTR2A Regulates Radial Glia Morphology.
(A) BrdU incorporation and Ki67-positive proliferating cells are not changed after HTR2A receptor activation or inhibition in cortical slices.
(B) Quantification of proliferation assay shown in (A).
(C) Treatment of cortical slices with HTR2A antagonist EMD 281014 leads to loss of radial fiber organization as visualized by vimentin staining and GFP-staining of cells infected with a CMV-GFP adenovirus.
(D) Higher magnification of inserts shown in (C) highlighting the lack of radial vimentin fiber organization after antagonist treatment.
(E) CMV-GFP-positive fibers in OSVZ are oRGs based on co-staining with Hopx.
(F and G) Quantification of fiber length reveals a significant reduction in average fiber length normalized by slice length (F) and fiber length normalized by slice length (G) in the EMD 281014-treated cortical slices compared to the control using the Wilcoxon rank-sum test. Data are represented as mean ± SEM. *p < 0.05, ****p < 0.0001.