KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Chicken anti GFP | Aves | GFP-1020; RRID: AB_10000240 |
| Goat anti Sox2 | Santa Cruz | sc17320; RRID: AB_2286684 |
| Mouse anti Ki67 | Dako | M7240; RRID: AB_2142367 |
| Mouse anti Vimentin | Sigma | V5255; RRID: AB_477625 |
| Rabbit anti HopX | Santa Cruz | sc30216; RRID: AB_2120833 |
| Alexa Fluor 546 donkey anti mouse | Life Technologies | A10036; RRID: AB_2534012 |
| Alexa Fluor 488 donkey anti chicken | Jackson | 703-545-155; RRID: AB_2340375 |
| Alexa Fluor 488 donkey anti mouse | Life Technologies | A-21202; RRID: AB_141607 |
| Alexa Fluor 546 donkey anti goat | Life Technologies | A-11056; RRID: AB_142628 |
| Alexa Fluor 647 donkey anti rabbit | Life Technologies | A31573; RRID: AB_2536183 |
| Rabbit polyclonal IgG Beta-III Tubulin | Covance | PRB-435P; RRID: AB_291637 |
| Rat anti Ctip2 | Abcam | ab18465; RRID: AB_2064130 |
| Mouse anti EOMES | Affymetrix | 14-4877-82; RRID: AB_2572882 |
| Streptavidin Dylight 549 | Vector Labs | SA-5549; RRID: AB_2336408 |
| Mouse anti Cryab | Abcam | ab13496; RRID: AB_300400 |
| Rabbit anti PPP1R17 | Sigma | HPA 047819; RRID: AB_2680166 |
| Chemicals | ||
| NMDA / Gly | Sigma | M3262 / G6761 |
| AMPA | Tocris | 169 |
| Acetylcholine | Sigma | A6625 |
| GABA | Sigma | A2129 |
| TCB-2 | Tocris | 2592 |
| MeSADP | Tocris | 1624 |
| Ionomycin | Sigma | I3909 |
| DNQX | Sigma | D0540 |
| EMD281014 | Tocris | 4470 |
| Suramin | Tocris | 1472 |
| D-APV | Tocris | 106 |
| Carbenoxolone | Tocris | 3096 |
| BMI | Sigma | B7561 |
| Oregon Green 488 BAPTA-1, AM | Invitrogen | O6807 |
| Critical Commercial Assays | ||
| RNAscope 2.5 HD Assay - RED | Advanced Cell Diagnostics | 322350 |
| Polaris Single-Cell Dosing mRNA Seq IFC | Fluidigm | PN 101–0082 |
| Deposited Data | ||
| RNA sequencing data | This paper | dbGaP: phs000989 |
| Software and Algorithms | ||
| ImarisXT | Bitplane | http://www.bitplane.com/ |
| GraphPad Prism 5 | GraphPad | https://www.graphpad.com/scientific-software/prism/ |
| Fiji (ImageJ) | NIH | https://fiji.sc/ |
| ggplot2 (R package) | https://cran.r-project.org/web/packages/ggplot2/index.html | |
| Fast Library for Approximate Nearest | Muja and Lowe, 2009 | https://www.cs.ubc.ca/research/flann/ |
| Neighbors (Python package) | ||
| signal (R package) | signal Developers, 2013 | https://cran.r-project.org/web/packages/signal/index.html |
| pracma (R package) | Borchers, 2018 | https://cran.r-project.org/web/packages/pracma/index.html |
| MAST (R package) | Finak et al., 2015 | https://www.bioconductor.org/packages/release/bioc/html/MAST.html |
| princurve (R package) | https://cran.r-project.org/web/packages/princurve/index.html | |
| cluster (R package) | https://cran.r-project.org/web/packages/cluster/index.html | |
| TrimGalore (Python package) | Kim et al., 2015 | http://www.bioinformatics.babraham.ac.uk/projects/trim_galore/ |
| Rsubread (R package) | Liao et al., 2014 | http://bioconductor.org/packages/release/bioc/html/Rsubread.html |
| MorphoLibJ (ImageJ plugin) | Legland et al., 2016 | https://imagej.net/MorphoLibJ |
| Shape Filter (ImageJ plugin) | Wagner and Lipinski, 2013 | https://imagej.net/Shape_Filter |
| Other | ||
| Pretreated German coverslips | Neuvitro | GG-12-Pre |
| Fibronectin | Sigma | F1141 |
| Matrigel | Corning | 354234 |