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. 2020 Jul 10;33(3):143–158. doi: 10.1007/s00497-020-00391-z

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© The Author(s) 2020

Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 3 — Developmental fate of exine-enclosed embryos and suspensor embryos. Cultures from LEC1:LEC1-GFP donor plants were immobilized on day five of culture and tracked for four days. For each set of panels, the light image is shown above and the CLSM image below. The green signal in the CLSM panels corresponds to LEC1-GFP expression. a An exine-enclosed embryo that develops into a suspensorless embryo. b An exine-enclosed embryo that develops into loose callus. c A few-celled suspensor embryo that continues to develop as a suspensor embryo. The suspensor can be distinguished from the embryo proper by the different division pattern of these cells; suspensor divisions are initially transverse, while the first embryo proper division is longitudinal. Labels: e, exine; sus, suspensor; ep, embryo proper