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. 2020 Nov 6;10:19232. doi: 10.1038/s41598-020-75301-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Cardiac phenotypes, mRNA expression AND Electrocardiogram (ECG) perturbations in cardiac tissue and effect of TLR 4 activation. (A) Heart weight to tail length ratio (HW/TL) (B) mRNA expression of ANP (C) heart rate in beats per minute (BPM) (D) R amplitude (E) QT duration. The mRNA expression data was normalized to the expression of reference gene, ribosomal protein L32 (RPL32). Data shown as Mean ± SEM, (N = 6 for HW/TL, N = 4 for mRNA expression and ECG) *p < 0.05, **p < 0.01 vs CON.