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. 2020 Nov 6;10:19232. doi: 10.1038/s41598-020-75301-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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mRNA expression after TLR4 activation in rat heart and in H9c2 (LPS 1 µg/mL) cells. mRNA expression of (A) IL-6 (B) TNF-α in rat heart tissue. mRNA expression of (C) IL-6 (D) TNF-α in H9c2 cells. (E) mRNA expression of TLR4, IL-6 and TNF-α in TLR4 overexpressed H9c2 cells (F) NFkB p65 protein expression in TLR4 overexpressed H9c2 cells. The data was normalized to the expression of reference gene, ribosomal protein L32 (RPL32). N = 4 for mRNA expression. *p < 0.05, **p < 0.01 vs CON group.