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. 2020 Nov 6;10:19232. doi: 10.1038/s41598-020-75301-4

Figure 6.

Figure 6

Apoptotic protein and gene mRNA expression in rat heart after TLR4 activation. (A) mRNA expression of p53, (B) mRNA expression of MDM2 (C) mRNA expression of BID (D) mRNA expression of BAD and (E) mRNA expression of caspase 9. (F) Protein expression of Cleaved caspase 7 and Cleaved caspase 9, (G) Fold change in expression of caspase 7, (H) Fold change in expression of caspase 9. (I) Apoptosis in H9c2 cells after TLR4 activation and overexpression. Whole gel stain was used for loading control for western blot. The mRNA expression data was normalized to the expression of reference gene, ribosomal protein L32 (RPL32). (N = 3 for western blot, N = 4 for mRNA expression). *p < 0.05, **p < 0.01 vs CON group.