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. 2020 Nov 6;10:19232. doi: 10.1038/s41598-020-75301-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Protein expression and acetylation of p53 in rat heart and effect of TLR 4 activation. (A) Protein expression of nuclear p53, (B) Fold change in expression of p53 (C) Protein expression of acetylated p53, (D) Fold change in expression of acetylated p53 (E) Protein expression and nuclear translocation of p53 in H9c2 cells after TLR4 activation. Whole gel stain was used for loading control for western blot. (G) Data shown as Mean ± SEM, (N = 4 for confocal analysis and N = 3 for western blot) *p < 0.05, **p < 0.01 vs CON.