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. 2020 Nov 6;10:19232. doi: 10.1038/s41598-020-75301-4

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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mRNA expression after TLR4 activation in rat heart. (A) mRNA expression of SIRT2, (B) Protein expression of SIRT2 (C) Fold change in mRNA expression of SIRT2 (D) Fold change in protein expression of SIRT2. Whole gel stain was used for loading control for western blot. The data for mRNA expression was normalized to the expression of reference gene, ribosomal protein L32 (RPL32). (E) NAD⁺/NADH ration after TLR4 activation, (F) SIRT2 activity in rat heart after TLR4 activation. (N = 4 for mRNA expression and SIRT2 activity, N = 3 for western blot). *p < 0.05, **p < 0.01 vs CON.