Fig. 2. Pathways and networks enriched in the genes with ASEs Pten^m3m4/m3m4 mouse brain.

a Top ten canonical pathways identified by Ingenuity Pathway Analysis (IPA) for P14 and P40 time points. b PTEN phenotype relevant “disease and bio function” networks identified by IPA at P14. From right to left: morphology of nervous system, development of central nervous system, and metabolism of carbohydrate. c Merged top networks from P14 and P40 with PTEN overlay and biological predictions using the Molecule Activity Predictor (MAP) tool on IPA. Green indicates the decrease in Pten expression observed in models with the m3m4 mutation. Orange molecules are predicted to be activated, while blue molecules are predicted to be inhibited. Edges that are orange are predicted activated, while those that are blue are inhibited, gray are without directional prediction, and yellow are inconsistent with the activity state of the connected node. The network is organized hierarchically so that higher nodes have regulatory influence over lower nodes. d Venn diagram of intersecting and separate genes with significant ASEs at P14 and/or P40.