Fig. 4. Evidence of isoform switching in Gfap, a molecular feature associated with astrocyte pathology.

a IGV snapshot of 3′ end of the Gfap locus focused on gap junction reads showing evidence of Gfapδ splicing event. b 3′ RACE sequencing of Gfap, showing representative clones from both Pten^m3m4 genotypes vs. wild type. c Isoform-specific qRT-PCR analysis for and Gfapδ. The ratio of the relative expression (i.e., 2^-(∆∆Ct)) of Gfapδ to Gfapα (Gfapδ/Gfapα) is graphed (*p < 0.05).