Skip to main content
. 2020 Nov 7;11(11):959. doi: 10.1038/s41419-020-03171-9

Fig. 4. Involvement of LINC00174 and SCD5 in lipid metabolism.

Fig. 4

A Dual luciferase assays in TC1889 cells using a vector enclosing SCD5 3′UTR or control vector (wt-3′UTR) in presence of miR-145-5p mimic oligonucleotide or control mimic. B, C Oil red assay to count lipid droplets in TC1889 cells upon LINC00174 (B) or SCD5 (C) silencing for 72 h (n = 4) (left). Quantification of lipid droplets was performed by imageJ software. Western blot analysis of Perilipin-2 protein, with relative quantification (n = 3), in TC1889 cells upon LINC00174 (B) or SCD5 (C) silencing for 72 h is shown on the right. Significant p-values are indicated as *P < 0.05; ***P < 0.0005; ****P < 0.00005.