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. 2020 Nov 7;11(11):959. doi: 10.1038/s41419-020-03171-9

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© The Author(s) 2020

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — A Transwell migration analysis to evaluate differences in motility upon SCD5 silencing (n = 3, p value = 0.0005) in TC1889 cells. Migration has been normalized over cell numbers in the indicated conditions. B RT-qPCR and C western blot analysis to verify silencing of SCD5 at transcript (n = 3, p value = 0.0003) and protein (n = 3, p value = 0.035) levels. D Confocal microscopy upon SCD5 silencing for 72 h after staining with phalloidine. E Transwell migration assay after 72 h of double or single depletion of LINC00174 and/or SCD5. Migration has been normalized over cell numbers in the indicated conditions. F Oil red assay for lipid droplets counting in the same conditions as in (E). Quantification of lipid droplets by imageJ software. Significant p-values are indicated as *P < 0.05; **P < 0.005; ***P < 0.0005; ****P < 0.00005.