Figure 2.

The effect of preincubation time and myo-inositol supplementation on the amount of mitochondria in mouse MII oocytes. A) MII oocytes preincubated in each simple and supplemented medium for 0, 4 and 8 hr were dyed with Mito Tracker Green to determine amount of mitochondria. Scale bar indicates 20 μm. B) The fluorescent intensity of MII oocytes stained by Mito Tracker Green. Fluorescent intensities of each stained oocyte were quantified with Image J software (ANOVA, p<0.001; Tukey’s post hoc, p<0.001)