Table 1.
Descriptive statistics of the oxidative stress, mitochondrial alteration, fertilization and developmental developmental rates in the mouse MII oocytes preincubated at different times and in different media
| Variables | No of oocytes | Control | 4 hr preincubaton | 8 hr preincubation | ANOVA/kruskal wallis p-value | ||
|---|---|---|---|---|---|---|---|
| Simple medium | Supplemented medium | Simple medium | Supplemented medium | ||||
| ROS level (pixel/oocyte) | 40 | 13.01±1.78 | 47.17±3.79 | 20.26±2.25 | 63.19±4.29 | 49.24±4.29 | ˂0.001 |
| GSH level (pixel/oocyte) | 40 | 77.29±3.57 | 49.39±4.76 | 67.67±4.28 | 16.73±2.65 | 43.41±5.87 | <0.001 |
| Amount of mitochondria (pixel/oocyte) | 40 | 80.16±1.64 | 60.34±4.63 | 79.90±4.99 | 44.90±6.04 | 57.00±2.81 | <0.001 |
| MMP (red/green ratio/oocyte) | 40 | 0.50±0.07 | 0.46±0.01 | 0.47±0.02 | 0.33±0.02 | 0.35±0.03 | <0.001 |
| ATP content (ng/ml ) | 45 | 106.25±10.33 | 109.58±14.01 | 141.41±6.21 | 106.08±15.10 | 159.41±9.64 | <0.001 |
| Fertilization rate | 100 | 84.64±4.02 | 78.90±1.11 | 82.95±3.55 | 66.92±2.66 | 77.23±2.02 | <0.001 |
| Cleavage rate | 32.05±0.64 | 25.44±1.64 | 31.35±1.12 | 16.92±1.16 | 18.89±0.68 | <0.001 | |
ROS: Reactive oxygen species, GSH: Glutathione, ATP: Adenosine triphosphate. Results are presented as mean±SD