FIGURE 4.

ROA regulates the secretion of adipokines of MSCs, especially PTX3 and IGFBP2. A and B, Adipokines in the culture supernatant were analyzed using a Human Adipokine Array Kit on day 5 of MSC adipogenesis after ROA knockdown. Differentially secreted adipokines are marked with colored boxes. Data were normalized to reference spots (n = 2). C, Relative expression of ANGPT1, ANGPT2, CFD, IGFBP2, and PTX3 determined by qRT‐PCR on day 3 of MSC adipogenesis after ROA knockdown/overexpression. D and E, Western blot of PTX3 and IGFBP2 on day 5. Data were normalized to GAPDH. The results are presented as the mean ± SD (n = 18, three independent experiments, each with six different samples unless stated elsewhere). *, P < .05; **, P < .01; ns, not significant, as determined by the Student's t‐test or ANOVA