Table 1. List of primers:
Gene | Forward 5'-3' | Reverse 5'-3' |
Spa | AGACGATCCTTCGGTGAGC | GCTTTTGCAATGTCATTTACTG |
Arc | TTGATTCACCAGCGCGTATTGTC | AGGTATCTGCTTCAATCAGCG |
aroE | ATCGGAAATCCTATTTCACATTC | GGTGTTGTATTAATAACGATATC |
Glp | CTAGGAACTGCAATCTTAATCC | TGGTAAAATCGCATGTCCAATTC |
Gmk | ATCGTTTTATCGGGACCATC | TCATTAACTACAACGTAATCGTA |
Pta | GTTAAAATCGTATTACCTGAAGG | GACCCTTTTGTTGAAAAGCTTAA |
Tpi | TCGTTCATTCTGAACGTCGTGAA | TTTGCACCTTCTAACAATTGTAC |
Yqi | CAGCATACAGGACACCTATTGGC | CGTTGAGGAATCGATACTGGAAC |
PVL | ATCATTAGGTAAAATGTCTGGACATGATCCA | GCATCAAGTGTATTGGATAGCAAAAGC |
mecA | GTAGAAATGACTGAACGTCCGATAA | CCAATTCCACATTGTTTCGGTCTAA |
Spa gene primers are specific for spa genotyping. Arc, aroE, Gip, Gmk, Pta, Tpi and Yqi genes primers are specific MLSA typing. MecA gene primers are specific for identification of MRSA. Luk gene primers are specific for identification of PLV strain. |