Fig. 2.

Evaluation of hybrid capture in mutant allele frequency profiling. A–C: Consistency among alternative allele frequencies (AAFs) in Raw, E1, and E2 data. Mutations were identified as described in the Materials and methods section; if a mutation was only identified in one of the comparison pairs, the AAF of the other one was used as long as its depth ≥ 10. D: Consistency between AAF changes from T1 to T2 in Raw and E1 data. Sites with depth ≥ 10, minor allele frequency ≥ 0.01, and minor allele supporting reads ≥ 2 were used. R² and p values of linear regressions are shown.