Table 2. ECFCs used per siRNA for testing siRNA efficiency and specificity.
Protein analysis | RNA analysis | ||
---|---|---|---|
siRNA | ECFC used to test siRNA efficiency | ECFC used to test siRNA specificity | ECFC used to test siRNA efficiency and specificity |
si1451A | C3 (1451AA) | C1 (1451GG) | C5 (1451AG) |
si1451G | C1 (1451GG) | C3 (1451AA) | |
si2365A | C3 (2365AA) | C2 (2365GG) | C5 (2365AG) |
si2365G | C2 (2365GG) | C3 (2365AA) | |
si2385T | C3 (2385TT) | C2 (2385CC) | C5 (2385TC) |
si2385C | C2 (2385CC) | C3 (2385TT) | |
si2880A | C4 (2880AA) | C1 (2880GG) | C5 (2880AG) |
si2880G | C1 (2880GG) | C4 (2880AA) |
Abbreviations: ECFC, endothelial colony-forming cell; siRNA, small interfering RNA.
Note: Genotype is indicated between brackets.