Table 6.
EC50 values of purified compounds isolated from ZP and TP propolis samples on T. congolense IL300, and a T. congolense cell line resistant to diminazene (n = 3).
| Purified Compounds | Trypanosoma congolense IL3000 | Trypanosoma congolense 6C3 | ||||
|---|---|---|---|---|---|---|
| EC50, AVG ± SEM (μg/mL) | EC50, AVG ± SEM (μM) | EC50, AVG ± SEM (μg/mL) | EC50, AVG ± SEM (μM) | RF | P | |
| (1) | 10.47 ± 0.96 | 32.30 ± 2.96 | 9.03 ± 0.43 | 27.85 ± 1.33 | 0.86 | 0.2430 |
| (2) | 7.35 ± 0.71 | 21.61 ± 2.08 | 5.69 ± 0.61 | 16.74 ± 1.79 | 0.77 | 0.1506 |
| (3 + 2) | 13.77 ± 1.26 | – | 12.94 ± 1.04 | – | 0.94 | 0.6383 |
| Diminazene | 0.15 ± 0.03 | 1.45 ± 0.04 | 9.81 | <0.0001 | ||
The EC50 values are the average and SEM of at least 3 independent determinations. RF= Resistance factor, being EC50(WT)/EC50(6C3). Statistical significance was determined using an unpaired two-tailed Student’s t-test comparing the EC50 values of the resistant strain with those obtained for the control strain Il3000.