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The standard curve of the DAS-qELISA. Two-fold serially diluted standard rPEDV-N protein with concentrations of 32, 16, 8, 4, 2, 1, and 0 μg/L was assayed using DAS-qELISA with two replicates per concentration. The standard curve was calculated using a linear relationship between the OD450 values and concentrations.
