FIGURE 2.

Proteasome inhibition did not rescue the decrease of AR expression. (A) Prostate cancer LNCaP (a) or 22RV1 (b) cells were treated with class I HDAC inhibitor MS-275 (1 μM) and/or protease inhibitor MG132 at indicated concentration for 48 h. Then total cell lysates were harvested and the expression of AR, maspin, and GAPDH was detected by WB. The level of GAPDH was used as equal loading control. (B) Prostate cancer LNCaP (a) or 22RV1 (b) cells were treated with demethylation agent 5′-Aza and/or protease inhibitor MG132 at indicated concentration for 48 h. Then total cell lysates were harvested and the expression of AR, maspin, and GAPDH was detected by WB. The level of GAPDH was used as equal loading control. (C) Overexpression of maspin clone 22RV1-M7 cells and the control clone 22RV1-Neo cells was treated with 5′-Aza and/or MG132 at indicated concentration for 48 h, respectively. Then total cell lysates were harvested and western blot was utilized to examine the expression of AR, maspin, and GAPDH. The level of GAPDH was used as equal loading control.