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. 2020 Nov 4;12:11257–11269. doi: 10.2147/CMAR.S270123

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© 2020 Li and Zheng.

This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php).

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MSI1 was a target of miR-125-5p. (A–D) The qRT-PCR and Western blot were implemented to assay the mRNA and protein levels of MSI1 in HCT116-R cells (A–B) and Nonresponders (C–D) with HCT116-P cells and Responders as respective controls. (E) The correlation between miR-125-5p and MSI1 was evaluated by Spearman correlation coefficient. (F) The binding sites of miR-125-5p in 3ʹUTR of MSI1 was predicted via Starbase 3.0. (G) The actual binding between miR-125-5p and MSI1 was affirmed using dual-luciferase reporter assay. *P < 0.05.