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. 2020 Nov 4;12:11271–11283. doi: 10.2147/CMAR.S255895

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© 2020 Zhu et al.

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CDK1 was knocked down by using siRNA.

Notes: (A and C) In HT-29 and Sw480 cell lines, the relative expression of CDK1 mRNA was examined by qRT-PCR in the situations of si-NC, siRNA1, siRNA2, and siRNA3. (B and D) In HT-29 and Sw480 cell lines, the relative expression of CDK1 protein was examined by WB in the situations of si-NC, siRNA1, siRNA2, and siRNA3. (E and F) CDK1 expression in fluorouracil-resistant cells significantly attenuated by using siRNA3. Quantity of protein was analyzed by Image J software. *P<0.05, **P<0.01, ***P<0.001.