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. 2020 Oct 26;10:512090. doi: 10.3389/fcimb.2020.512090

Table 1.

Homemade methods used for titration antibodies directed against F. tularensis antigens.

Method F. tularensis strain Antigen References
Homemade tests
TAT LVS (BB IND 157) formalin-inactivated bacteria (Koskela and Salminen, 1985)
MAT Ootake tick strain formalin-inactivated and safranine-stained bacteria (Sato et al., 1990)
LVS (ATCC 29648) methanol-fixed bacteria (Porsch-Ozcürümez et al., 2004)
LVS (BB IND 157) formalin-inactivated bacteria (Syrjälä et al., 1986)
hare type B strain formalin-inactivated and safranine-stained bacteria (Bevanger et al., 1988)
human type B strain NA (Helvaci et al., 2000)
LVS formalin-inactivated and safranine-stained bacteria (Splettstoesser et al., 2010)
LVS formalin-inactivated and safranine-stained bacteria (Kiliç et al., 2012)
LVS formalin-inactivated bacteria (Chaignat et al., 2014)
LVS (NCTC 10857) formalin-inactivated bacteria (Yanes et al., 2018)
LAT Ft formalin-inactivated and sonicated bacteria (Rastawicki et al., 2015)
IFA LVS (ATCC 29648) methanol-fixed bacteria (Porsch-Ozcürümez et al., 2004)
human type B strain formalin-inactivated bacteria (Maurin et al., 2011)
ELISA LVS Lipopolysaccharide extract (Carlsson et al., 1979)
NA formalin-inactivated and sonicated bacteria (Viljanen et al., 1983)
LVS (BB IND 157) formalin-inactivated and sonicated bacteria (Koskela and Salminen, 1985)
LVS (BB IND 157) Bacterial sonicate (Syrjälä et al., 1986)
LVS (ATCC 29648) Lipopolysaccharide extract (Porsch-Ozcürümez et al., 2004; Schmitt et al., 2005)
LVS (ATCC29684) Lipopolysaccharide extract (Chaignat et al., 2014)
CF-ELISA LVS (BB IND 157) formalin-inactivated and sonicated bacteria (Koskela and Salminen, 1985)
cELISA hare type B strain (NVF1) Lipopolysaccharide extract (Sharma et al., 2013)
ICT NA Lipopolysaccharide extract (Micromun ®) (Splettstoesser et al., 2010)

TAT, tube agglutination test; MAT, microagglutination tests; LAT, latex agglutination test; IFA, immunofluorescence assay; ELISA, enzyme-linked immunosorbent assay; CF-ELISA, complement-fixing ELISA; cELISA, competitive ELISA; ICT, immunochromatographic assay; LVS, live vaccine strain.