Fig 1.
Preferential homing of T cells to the skin in patients with OS. A, Representative FACS plots and frequencies of CLA+ CD45RO+ T cells within the effector CD4+ and CD8+ T-cell populations isolated from PB of patients with OS (n = 13) and aged-matched HDs (n = 31). Frequencies of CCR4+ within the CD4+ CD45RA− T-cell population (n = 11-15). Plasma levels of chemokines (B) and cytokines (C) in HDs (n = 37), in patients with OS (n = 15) as well as in patients with acute graft-versus-host disease (GvHD) (n = 15) and patients with AD (n = 14), as controls of Th1- and Th2-mediated inflammation, respectively. D, Skin biopsies from patients with OS were investigated for IFNG, CXCL9, and CXCL10 mRNA expression using RNAscope. POLR2A mRNA labeling was used as control for the quality of the RNA on paraffin section. Values are mean ± SEM. ∗P < .05, ∗∗P < .01, ∗∗∗P < .005, ∗∗∗∗P < .001. Statistical significance determined by Mann-Whitney U test or by ANOVA. See also Table E1.