Fig 4.
Characterization of cutaneous inflammatory environment in Rag2R229Q mice. A, Mean fluorescence intensity (MFI) for MHCII expression among CD45− population of skin suspension (n = 6-7 mice/group). Transcriptional analysis of several cytokines (B) and chemokines (C) in the skin tissue (n = 6-9 mice/group from 3 experiments). Target mRNA was normalized to Actb mRNA. RNA contents are shown as AUs. D, Levels of the chemokines Ccl17 and Ccl22 in the serum of Rag2+/+ and Rag2R229Q mice (n = 12-13 mice/group from 3 experiments). E, Representative FACS plots and cumulative frequencies of CCR4+ inside the CD3+ T-cell population of spleen, MLN, and DLN (n = 14 mice/group from 3 experiments). Values are mean ± SEM. ∗P < .05; ∗∗P < .01; ∗∗∗P < .005; ∗∗∗∗P < .001. Statistical analysis was performed using Mann-Whitney U test. See also Fig E3. SSC-A, Side scatter.