Fig 7.

Boosted intestinal inflammation worsens skin inflammation in Rag2^R229Q mice. A, Colitis in Rag2^R229Q mice was induced by cyclic DSS administration. Representative skin sections stained with H&E and CD3 immunostaining. Bars = 200 μm (H&E) and 100 μm (CD3). Higher magnification shows eosinophils. Bar = 50 μm. Histogram shows the inflammation score in the skin (n = 12-15 mice/group from 3 experiments). B, Representative skin sections from untreated and DSS-treated Rag2^R229Qmice stained with CD31 immunostaining (asterisks mark the CD31-positive vessels). Bar = 100 μm. Correlation between cutaneous inflammation and average vessels size of untreated Rag2^R229Q (black dots) and DSS-treated Rag2^R229Q (blue dots). The Spearman r value is indicated in the graph (n = 5 mice/group). C, Fold change of the CD4⁺ and CD8⁺ T-cell frequencies (n = 6-9 mice/group). D, Fold change of cutaneous Cxcr3, Cxcl10, Ifng, Ccl20, Tslp, and Tlr4 expressions (n = 6-9 mice/group). E, MFI for MHCII expression among CD45⁻ population of Rag2^R229Q and DSS-treated Rag2^R229Q skin suspension (n = 5-6 mice/group). F, Representative FACS plots and cumulative frequencies of splenic Ccr4⁺, Ccr9⁺, and Ccr4⁺Ccr9⁺ CD4⁺ T cells (n = 6-11 mice/group from 3 experiments). Dotted line indicates WT averages. Values are mean ± SEM. ∗P < .05; ∗∗P < .01; ∗∗∗P < .005. Statistical significance determined by Mann-Whitney U test. See also Fig E4.