Fig E7.

Effect of DSS-induced intestinal inflammation on experimental model of AD. Mice were treated with MC903 for 8 days to induce AD-like dermatitis. Starting on day 8, a set of mice received 2% DSS water for 7 days, and then returned to normal drinking water for additional 2 days. A, Plasma LBP on day 17 is shown. B, Day 17 gross phenotype of AD mice treated or not with oral DSS. Bar graph shows the difference in ear thickness between day 17 and day 8 (n = 5-10 mice/group). C, Representative ear section from AD and AD+DSS mice stained with H&E (left; bar = 500 μm) and CK5 immunostaining (right; bar = 200 μm). Histogram shows the inflammation score in the ear skin (n = 5-10). D, Gene expression analysis of the ear tissue (n = 5-10 mice/group). Target mRNA was normalized to Actb mRNA. RNA contents are shown as AUs. Values are mean ± SEM. ∗P < .05. Statistical analysis was performed using Mann-Whitney U test.