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. 2020 Oct 20;23(11):101712. doi: 10.1016/j.isci.2020.101712

Figure 5.

Figure 5

Curvature Sensing of IDRFBP17L, F-BAR, and F-BAR + IDR on Nanobar Array

(A) SEM image of nanobar arrays in width gradient from 200 to 600 nm with 100-nm step. Every two columns of the arrays are the same size as indicated. The height of every nanobar is 600 nm. Scale bar, 5 μm.

(B–F) Diagrams (top) and averaged images (middle) of lipid bilayer (B), 16 μM IDRFBP17L (C), 25 μM IDRCIP4 (D), 16 μM F-BAR (E), and 16 μM F-BAR + IDR (F) on nanobars with width from 200 nm to 600 nm. Each image was averaged from over 60 nanobars. Scale bar, 2 μm. Bottom: Intensity profiles along nanobar of each averaged image. Scale bars: 10,000, 2,000, 10, 2,000, 5,000 a.u. in (B) to (F).

(G) Normalized nanobar-end density of FBP17 F-BAR, IDR, and F-BAR + IDR based on their corresponding lipid bilayer intensity. Each point represents mean ± SEM from over 60 (n = 3 experiments per protein).