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Alloreactive T cell clones differentially recognize allogeneic BLCs. Representative data from T cell clone 11 demonstrating strong reactivity against two of the ten allogeneic BLCs. Equal numbers of T cells and BLCs were incubated overnight in an IFN-γ ELISpot assay. (+) and (−) signify animals that were placed into mHAgpos and mHAgneg groups for WES segregation analysis, respectively. SFC, spot-forming cell.
