FIGURE 2.

An mHAg-reactive T cell clone recognizes an amino acid variant in APOBEC3C. (A) T cell clone T11 was incubated with mHAg^neg BLCs from cy0736 (left) or cy0746 (right) pulsed with overlapping 15-mer peptides containing an arginine-to-leucine amino acid change relative to the mHAg^neg sequence in an IFN-γ ELISpot assay. (B) Sequences of the APOBEC3C (A3C) peptides used to pulse the mHAg^neg BLCs in panel (A). The variant amino acid is highlighted in bold. The gray box corresponds to the putative region containing the mHAg epitope. (+) and (−) signify wells determined to be positive or negative, respectively. SFC, spot-forming cell.