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. 2020 Oct 26;11:586251. doi: 10.3389/fimmu.2020.586251

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Copyright © 2020 Weinfurter, Graham, Ericsen, Matschke, Llewellyn-Lacey, Price, Wiseman and Reynolds.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Tetrameric complexes of Mafa-A1*063/SW10 stain APOBEC3C SW10-specific CD8⁺ T cells. An APOBEC3C mHAg-specific T cell line was stained with PE-conjugated tetrameric complexes of Mafa-A1*063/SW10. Displayed is the flow cytometric gating strategy for data analysis, progressively selecting singlets, live cells, CD3⁺, and CD8⁺/tetramer⁺ cells.